Buffers
Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesize DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and for Loop-Mediated Isothermal DNA Amplification (LAMP).
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High Conc. Glycerol-Free Bst, MDX018
High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).
Documents & Resources
Bst Reaction Buffer, 10x, MDX076
Reaction Buffer optimized for use with Bst DNA Polymerase.
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Enzyme Dilution Buffer, 1x, MDX078
Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration.
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Enzyme Dilution Buffer, 10x (Glycerol free), MDX080
Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.
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Inhibitor-Tolerant Bst Buffer, MDX019
Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions.
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Catalogs & Brochures
Isothermal amplification such as loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that can be performed at a single temperature. LAMP is currently considered one of the most powerful isothermal amplification techniques, relying on a strand-displacement polymerase combined with four to six primers. These primers recognize several specific regions in the target DNA and two of the primers form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA.
Meridian’s Bst polymerases have strong strand displacement activity, fast polymerization, and enhanced inhibitor and salt tolerance when used with the specialized Inhibitor-Tolerant reaction buffer. The 100x high-concentration, glycerol-free BST enzyme was specifically designed to maximize assay flexibility for LAMP diagnostic test development.
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